Jumbo Frame has be set end-to-end to take into effect.
1. Jumbo Frame at vmkernel nic can be setting only at creating, not modifiable later
esxcfg-vmknic -a myvmknic -i x.x.x.x -mtu 9000 <port_group>
2. Jumbo Frame Virtual Switch level for vSS
esxcfg-vswitch -m 9000 <vSwitch>
3. Jumbo Frame for vDS, could be set from vSphere client interface.
4. Jumbo Frame for VM level
Enhanced vmxnet is used for enabling Jumbo Frame at VM settings
5. Jumbo Frame at guest OS level.
Inside Guest OS, configure the network adapter to allow Jumbo Frames
Linux Red Hat: ifconfig eth0 mtu 9000 (MTU 9000 edit /etc/sysconfig/network-scripts/ifcfg-eth0 to make changes permanent)
Windows: NIC property->advanced->Jumbo Frame->9000
6. Application Protocol Tunning.
update existing NFS, SMB, iSCSI for Jumbo Frame.
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